UMB 4: Assessment of Single and Combined Effects of Environmental Stressors on The Mitochondrion Using Fish Cells, Crustaceans, Algae and Plants
Y. Song (NIVA), M.T. Hultman (NIVA/NMBU-IMV), T. Gomes (NIVA), L. Fredriksen (NIVA), L. Xie (NIVA), K.E. Tollefsen (NIVA/NMBU-IMV).
Objective: To develop and utilize a bioassay to study the mitochondrial dysfunction and oxidative stress caused by environmental stressors.
Results: A fluorescence-based bioassay was used in this study for rapid measurement of disruption of mitochondrial inner membrane potential. The assay has been successfully optimized for two types of fish cells, including zebrafish (Danio rerio) liver cell-line (ZF-L) and Arctic char (Salvelinus alpinus) primary hepatocytes, the green algae (Chlamydomonas reinhardtii), the duckweed (Lemna minor) and the water flee (Daphnia magna). Assays with ZF-L cell line showed clear inhibition of MMP by the classical uncoupler carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and cadmium.
Project Manager: You Song
A fluorescence microscopic image of the waterflee Daphnia magna after 24h exposure to 1000 nM carbonyl cyanide 3-chlorophenylhydrazone (CCCP). Orange color indicates the fluorescence signal of TMRM. (Figure: Y. Song, NIVA).