UMB 5: Characterising and Quantifying Epigenetic Changes that have Relevance for Transgenerational Effects in Daphnia Magna after Exposure to Single and Multiple Stressors
J. Thaulow (NIVA), L.C. Lindeman (NMBU-NVH), J.T. Rundberget (NIVA), Peter Aleström (NMBU-NVH), Knut Erik Tollefsen (NIVA/NMBU-IMV).
Objectives: Establish toolbox for determining epigenetic changes in D. magna.
Methods: LC-MS/MS - simultaneous quantification of different methylation forms ChIP– elucidate changes at histone modifications on gene promoters.
- Detection of 5-methylcytosine – 5meC (standard epigenetic marker).
- Detection of it’s oxidized form 5-hydroxymethylcytosine – 5hmC.
- 5hmC potentially more biological relevant than 5meC
- Establishment of a ChIP-PCR protocol for Daphnia magna.
- High enrichment of H3K9me3 and H3K27me3 in promotor associated with transcriptional repression.
Conclusions: Methods promising for future epigenetic work in Daphnia magna, and other non-model organisms.
Project Manager: Jens Thaulow