Caged blue mussels as environmental detectives
May 2015, Kristiansand, Norway. Two researchers in a boat loaded with thousands of blue mussels, collected from a mussel farm in Lillesand. The boat heads out the Kristiansand fjord, and the researchers deploy the blue mussels in the sea. Why are they doing this?
Blue mussel. This shellfish has its home in the intertidal areas in the sea, where it pumps large volumes of sea water over its ciliated gills. The blue mussel filtrates phytoplankton and pollutants from the water, takes up the plankton as food, and stores the pollutants in its tissues. The filtration and storage of pollutants are so efficient, that blue mussels are used in environmental monitoring; they are like environmental detectives. But, to say something certain about the pollution level in the fjord, a lot of blue mussels is needed; and picking massive amounts in areas where there are only few mussels, or even no mussels at all, is impossible. Now the researchers are experimenting with caged mussels: can newcomer mussels replace native mussels in environmental monitoring?
Caged for half a year
This was what the researchers from the Norwegian Institute for Water Research (NIVA) wanted to find out, as they were driving in the fjord in the boat full of blue mussels. The Kristiansand harbour was perfect for the experiment: Years of metal industry had contaminated the fjord with nickel, copper, and cobalt, in addition to the organic compounds PAH (polycyclic aromatic hydrocarbons), PCB (polychlorinated biphenyls), and HCB (hexachlorobenzene). Two years ago, there were only few naturally occurring blue mussels in the harbour, whilst there were plenty further out in the fjord off Svensholmen. The researchers deployed the two cages in the fjord, one close to the harbour and one by Svensholmen, each containing one thousand clean blue mussels from the mussel farm in Lillesand.
- We collected mussels from the cages and from the natural population several times during the following six months, Merete Schøyen, the NIVA researcher responsible for the blue mussel experiment, says.
- Then we brought the mussels to the lab in Oslo for analysis and comparison of contaminant levels.
NIVA-scientists have compared caged and native blue mussels (Mytilus edulis spp.) for environmental monitoring of PAH, PCB and trace metals (Photo: Lise Tveiten, NIVA).
35 times as much nickel
When the test results were ready, there was no doubt the water masses outside Kristiansand were polluted. Six months behind bars had increased the levels of both metals, PAHs, PCBs, and HCBs in the blue mussels. In the cage deployed closest to the harbour, the concentration of nickel had increased up to 35 times compared to the pre-deployed mussels. But increased levels in itself were not what interested the researchers: They wanted to study how the pollutant levels developed over time, and establish how long it took for each pollutant group to reach steady state levels.
Metal levels increased faster than organic contaminants
- After only one month, metal concentrations in the farmed mussels were at the same levels as in the natural population at Svensholmen, Schøyen says.
- However, accumulation of the organic compounds was slower. After six months, the levels of PAHs and PCBs were still higher in the natural population than in the caged mussels.
These findings indicate how long the blue mussel cage should stay in the fjord to reach representative levels of pollutants. For use in environmental monitoring, the deployment time should be as short as possible, and a half year – or even more – is not practical. But when combined with so-called toxicokinetic models, it might be possible to estimate the pollutant levels in local, natural blue mussels, using caged mussels which have been deployed only a short period.
National monitoring standards
The blue mussel experiment resulted in two scientific articles about the use of caged mussels in environmental monitoring, published in Marine Environmental Research. The articles also highlight the need of more research, for instance about annual variation in contaminant levels, to improve the quality of the monitoring data. In collaboration with the Norwegian Environment Agency and Standards Norway, the NIVA researchers are developing a national standard for the monitoring of contaminants in blue mussels.
This study was funded by the Norwegian Research Council through NIVA's institutional funding and the Norwegian Environment Agency via the National Function programme.
Reference: Schøyen, M., Allan, I.J., Ruus, A., Håvardstun, J., Hjermann, D.Ø., Beyer, J. (2017) Comparison of caged and native blue mussels (Mytilus edulis spp.) for environmental monitoring of PAH, PCB and trace metals, Marine Environmental Research, http://dx.doi.org/10.1016/j.marenvres.2017.07.025. Beyer, J., Green, N.W., Brooks, S., Allan, I.J., Ruus, A., Gomes, T., Bråte, I.L.N., Schøyen, M. (2017) Blue mussels (Mytilus edulis spp.) as sentinel organisms in coastal pollution monitoring: A review. Marine Environmental Research. http://dx.doi.org/10.1016/j.marenvres.2017.07.024
Schøyen, M., Allan, I.J., Ruus, A., Håvardstun, J., Hjermann, D.Ø., Beyer, J. (2017) Comparison of caged and native blue mussels (Mytilus edulis spp.) for environmental monitoring of PAH, PCB and trace metals, Marine Environmental Research, http://dx.doi.org/10.1016/j.marenvres.2017.07.025.
Beyer, J., Green, N.W., Brooks, S., Allan, I.J., Ruus, A., Gomes, T., Bråte, I.L.N., Schøyen, M. (2017) Blue mussels (Mytilus edulis spp.) as sentinel organisms in coastal pollution monitoring: A review. Marine Environmental Research. http://dx.doi.org/10.1016/j.marenvres.2017.07.024