Realistic pesticide exposure through water and food amplifies long-term effects in a Limnephilid caddisfly

Summary

Pesticides are increasingly recognized as relevant stressors in stream ecosystems. Stream biota is exposed to pesticides with low water solubility, e.g. pyrethroid insecticides, via water, habitat, and food. However, long-term effects of simultaneous exposure pathways are unknown. In this context, we conducted a microcosm experiment with the caddisfly Anabolia nervosa exposing the larvae to the pyrethroid insecticide esfenvalerate (EFV) at 0.1 and 1.0 μg L− 1 via (i) water, (ii) food or a (iii) combination of water and food. Combined exposure through water and food significantly reduced emergence by 60% and significantly postponed emergence timing at the highest EFV level, whereas none of the single-phase exposures showed significant effects. Moreover, our study revealed that successfully emerged females from the highest biphasic treatment level were characterised by altered composition of storage lipids indicative of reduced energy reserves. Consequently, a realistic test scenario that represents simultaneous exposure of organisms and their food may reveal substantially increased long term effects of pyrethroids when compared with current ecological risk assessment applying only single phase exposure. We recommend that relevant concurrent exposure routes of pesticides should be considered in order to derive realistic regulatory acceptable concentrations of pesticides.